The iLite® Reagent BLANK should be used in the anti-TNF-alpha NAb assay. It can be used for the preparation of the blank sample and also for the dilution of TNF-alpha inhibitors.
Application Notes for the following assays are available:
• Determination of neutralizing antibodies against TNF-alpha blockers using iLite® TNF-alpha Assay Ready Cells (E174-GB)
Tumor necrosis factor alpha (TNF-alpha) promotes inflammatory responses, which, in turn, contribute to the clinical problems associated with many inflammatory disorders, including rheumatoid arthritis, ankylosing spondylitis, Crohn's disease, psoriasis and refractory asthma. These diseases are sometimes treated with TNF-alpha inhibitors, including infliximab, adalimumab, etanercept, certolizumab pegol or golimumab. Prolonged therapies with these TNF-alpha inhibitors may lead to development of neutralizing antibodies (NAbs), which may counteract the TNF-alpha antagonist activity of the drugs.
0.2 mL of iLite® Reagent BLANK containing RPMI 1640 with 30% heat inactivated fetal bovine serum (FBS).
